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Updated: Aug 13, 2026

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
Published on: March 17, 2010
Alkyl isocyanates as active site-specific inhibitors of chymotrypsin and elastase
Abstract:
Alkyl isocyanates react specifically with the two serine proteinases, chymotrypsin and elastase, to yield inactive enzyme derivatives containing 1 male of reagent per mole of enzyme. Octyl isocyanate inactivates chymotrypsin only, while butyl isocyanate inactivates both enzymes but shows greater efficiency toward elastase than toward chymotrypsin. These reagents may thus represent unique chemical "yardsticks" for the measurement of the relative dimensions of the active sites of the two very similar enzymes.
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