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Updated: Aug 13, 2026

Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo
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Paramyxoviruses for Tumor-targeted Immunomodulation: Design and Evaluation Ex Vivo

Published on: January 7, 2019

The vectorial release of nascent immunoglobulin peptides

M J Bevan

    The Biochemical Journal
    |March 1, 1971
    PubMed

    Insights

    This study investigated nascent immunoglobulin peptide release from mouse plasmacytoma cells. Results show that newly synthesized immunoglobulin chains remain associated with microsomal vesicles after release from ribosomes.

    Area of Science:

    • Molecular Biology
    • Cell Biology
    • Immunology

    Background:

    • Mouse plasmacytoma MOPC 47A cells secrete immunoglobulin A (IgA).
    • Studying nascent peptide release requires methods to distinguish newly synthesized proteins from completed ones.

    Purpose of the Study:

    • To investigate the release of nascent immunoglobulin peptides in vitro.
    • To determine the association of nascent immunoglobulin chains with microsomal vesicles.

    Main Methods:

    • Utilized a microsomal preparation from mouse plasmacytoma MOPC 47A.
    • Employed puromycin to release nascent peptide chains.
    • Used radiolabeling with [(3)H]leucine and [(3)H]-puromycin for detection.
    • Characterized released peptides using specific antiserum against tumor immunoglobulin.

    Main Results:

    • Experiments with [(3)H]leucine showed up to one-third of released radioactivity was immunoglobulin, but complicated by background.
    • Experiments with [(3)H]-puromycin provided clearer interpretation, identifying up to one-tenth of released radioactivity as immunoglobulin.
    • Both labeling methods demonstrated that all recognizable nascent immunoglobulin chains remain associated with microsomal vesicles post-ribosomal release.

    Conclusions:

    • Nascent immunoglobulin chains are retained within microsomal vesicles after synthesis.
    • This association is crucial for proper immunoglobulin processing and secretion.

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