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Improved recognition and quantitation technique for oncornaviruses.

R Stephens, K Traul, D Woolf

    Journal of Clinical Microbiology
    |February 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

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    A new method using perforated BEEM capsules accurately counts oncornaviruses, even those difficult to analyze with traditional methods. This reliable technique enhances virus quantification sensitivity and precision.

    Area of Science:

    • Virology
    • Microscopy techniques

    Background:

    • Accurate quantification of oncornaviruses is crucial for understanding viral diseases.
    • Traditional methods like negative staining can be challenging for certain virus samples.

    Purpose of the Study:

    • To develop and validate a novel technique for recognizing and quantifying oncornaviruses.
    • To improve upon existing methods for virus sample analysis.

    Main Methods:

    • Development of a thin-sectioning technique utilizing perforated BEEM capsules.
    • Application of the technique to virus samples problematic for negative staining.
    • Serial dilutions of viral concentrates to determine technique sensitivity.

    Main Results:

    • The BEEM capsule technique enabled clear definition and counting of previously difficult virus samples.

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  • Perforation facilitated rapid reagent infiltration, simplifying sample preparation.
  • Sensitivity was established at 5 x 10^5 virus particles/ml.
  • High precision was demonstrated with repeat counts varying only +/- 0.3 log.

    • Conclusions:

    • The perforated BEEM capsule method offers a reliable and sensitive approach for oncornavirus quantification.
    • This technique overcomes limitations of negative staining, improving diagnostic capabilities.
    • The method's precision and ease of use make it a valuable tool in virology research.