Investigation of the lethal yellow Ay/Ay embryo using mouse chimaeras

Chimaeric combinations of normal and mutant embryonic tissues were used to investigate the lethal effect of the yellow gene. The homozygous mutant embryos could not be identified before implantation. Therefore, embryos from both intercross matings and control backcross matings were used to provide inner cell masses (ICMs) for injection into genetically marked blastocysts of the CFLP random bred stock. All conceptuses obtained from reimplanted blastocysts were analysed at mid-gestation for the presence of donor isozyme of glucose phosphate isomerase. A similar proportion of chimaeras were found in the experimental and control series, indicating rescue of the lethal Ay/Ay ICM tissue. The reciprocal experiment also produced a similar proportion of chimaeras but there was a 25% postimplantational loss of injected embryos evidenced by empty decidual swellings. The results suggest that the yellow mutation primarily affects the trophectoderm which cannot be rescued by a normal ICM, whereas Ay/Ay ICM is capable of survival in a chimaera at least until mid-gestation.