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Some staining characteristics of Mott's or morular cells of nagana
Beitrage Zur Pathologie
|August 1, 1975
Abstract:
The spherules of morular (Mott's) cells are not metachromatic with toluidin blue at pH 8 and the majority are stained yellow with PTAH. This distinguishes them from globule leucocytes. They differ from Russell bodies of plasma cells which are Gram-positive.
Insights
Morular (Mott's) cells contain spherules that do not stain with toluidine blue and are yellow with PTAH, differentiating them from globule leukocytes. These spherules are distinct from Russell bodies, which are Gram-positive.
Area of Science:
- Cytology and Histopathology
- Cell Biology
Background:
- Morular (Mott's) cells are characterized by cytoplasmic spherules.
- Distinguishing these spherules from similar cellular inclusions is crucial for accurate diagnosis.
Purpose of the Study:
- To differentiate the spherules of morular (Mott's) cells from globule leucocytes and Russell bodies using specific staining techniques.
- To establish reliable histological criteria for identifying morular (Mott's) cell spherules.
Main Methods:
- Histochemical staining with toluidine blue at pH 8.
- Histochemical staining with PTAH (Phosphotungstic acid-hematoxylin).
- Gram staining.
Main Results:
- Morular (Mott's) cell spherules demonstrated a lack of metachromasia with toluidine blue at pH 8.
- The majority of morular (Mott's) cell spherules stained yellow with PTAH.
- Globule leucocytes and Russell bodies exhibited different staining characteristics, aiding differentiation.
Conclusions:
- The differential staining patterns with toluidine blue and PTAH effectively distinguish morular (Mott's) cell spherules from globule leucocytes.
- Morular (Mott's) cell spherules are histologically distinct from Russell bodies, which are Gram-positive.