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In Vivo Forward Genetic Screen to Identify Novel Neuroprotective Genes in Drosophila melanogaster
Published on: July 11, 2019
Transplantation of nucle in Drosophila melanogaster
Abstract:
Nuclei surrounded by ooplasm of the syncytial stage of developing eggs of wild-type Drosophila melanogaster were implanted into freshly laid fertilized eggs of females of a y w stock. More than half of the recipient eggs produced larvae, but few of the larvae hatched or developed further. The best sets of experiments gave about twelve percent of imagos, mostly y w in appearance. Several larvae were mosaics with yellow Malpighian tubes, and two flies had part of the abdominal segments of the wild type. Half of the flies were fertile, but they produced only y w offspring, except for two males that had y w appearance, but wild-type gonads. When crossed with y w females, they gave wild-type females and y w males.

