Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Initial dipeptide formation in hemoglobin biosynthesis.

R G Crystal, D A Shafritz, P M Prichard

    Proceedings of the National Academy of Sciences of the United States of America
    |August 1, 1971
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Influence of Diurnal Temperatures on the Susceptibility of Bedding Plants to Gray Mold Caused by Botrytis cinerea.

    Plant disease·2019
    Same author

    Search for lepton flavour violation in the <i>eμ</i> continuum with the ATLAS detector in [Formula: see text]<i>pp</i> collisions at the LHC.

    The European physical journal. C, Particles and fields·2015
    Same author

    Search for new phenomena in tt events with large missing transverse momentum in proton-proton collisions at sqrt[s] = 7 TeV with the ATLAS detector.

    Physical review letters·2012
    Same author

    Search for dilepton resonances in pp collisions at √s=7 TeV with the ATLAS detector.

    Physical review letters·2012
    Same author

    Search for a standard model Higgs boson in the H→ZZ→ℓ(+)ℓ(-)νν decay channel with the ATLAS detector.

    Physical review letters·2011
    Same author

    Search for the Higgs boson in the H→WW→lνjj decay channel in pp collisions at √s=7 TeV with the ATLAS detector.

    Physical review letters·2011
    Same journal

    Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Tomographic imaging of superconducting order using particle-hole interference.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Analytical modeling for suction cup designs for skin-interfaced wearable devices.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    Same journal

    Improving cell-free metabolism through direct integration of artificial respiratory chains.

    Proceedings of the National Academy of Sciences of the United States of America·2026
    See all related articles

    Rabbit reticulocyte ribosomes utilize Met-tRNA(F) as the initiator tRNA for hemoglobin biosynthesis. Initiation factor M(3) is crucial for the first peptide bond formation but not for initial Met-tRNA(F) binding.

    Area of Science:

    • Molecular Biology
    • Protein Synthesis
    • Biochemistry

    Background:

    • Hemoglobin biosynthesis is a fundamental process in red blood cell development.
    • Understanding the initiation of protein synthesis is key to deciphering gene expression regulation.

    Purpose of the Study:

    • To identify the specific initiator tRNA for hemoglobin synthesis in rabbit reticulocytes.
    • To elucidate the roles of initiation factors in the formation of the first peptide bond of hemoglobin.

    Main Methods:

    • Ribosome binding assays using rabbit reticulocyte ribosomes and labeled Met-tRNA.
    • Analysis of dipeptide synthesis (methionyl-valine) in the presence and absence of specific initiation factors and tRNAs.

    Main Results:

    Related Experiment Videos

  • Met-tRNA(F) (formyl-methionyl tRNA) binds to the small ribosomal subunit, initiating hemoglobin synthesis.
  • Formation of the methionyl-valine dipeptide requires Met-tRNA(F), initiation factors M(1), M(2), M(3), Val-tRNA, and T(1).
  • Met-tRNA(M) (methionyl-tRNA) cannot substitute for Met-tRNA(F), and omission of M(3) prevents dipeptide synthesis.

    • Conclusions:

    • Met-tRNA(F) is confirmed as the initiator tRNA for hemoglobin biosynthesis.
    • Initiation factor M(3) is essential for the synthesis of the first peptide bond, but not for the initial binding of Met-tRNA(F) to the ribosome.