Purification and characterization of Clostridium botulinum type B oxin

Gerwing, Julia (The University of British Columbia, Vancouver, B.C., Canada), Claude E. Dolman, David V. Kason, and Jack H. Tremaine. Purification and characterization of Clostridium botulinum type B toxin. J. Bacteriol. 91:484-487. 1966.-A toxic component of low molecular weight has been isolated from a type B strain of Clostridium botulinum by methods involving ammonium sulfate precipitation and elution through diethylaminoethyl cellulose at pH 5.6. The material thus isolated was shown to be monophoretic and monodisperse in the ultracentrifuge. End-group analysis indicated the presence of a single N-terminal amino acid residue, which was identified as arginine. On the basis of biophysical studies and amino acid analyses, a molecular weight between 9,000 and 10,000 was calculated.