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Nucleotide sequence of tomato ringspot virus RNA-2
M E Rott1, J H Tremaine, D M Rochon
1Department of Plant Science, University of British Columbia, Vancouver, Canada.
The Journal of General Virology
|July 1, 1991
Summary
The tomato ringspot virus (TomRSV) RNA-2 sequence was determined, revealing a long open reading frame (ORF) encoding coat proteins. Limited sequence similarity was found with other nepoviruses, particularly in the coding region.
Area of Science:
- Virology
- Molecular Biology
- Genomics
Background:
- Tomato ringspot virus (TomRSV) is a significant plant pathogen.
- Understanding viral RNA sequences is crucial for comprehending viral replication and pathogenesis.
Purpose of the Study:
- To determine the complete nucleotide sequence of TomRSV RNA-2.
- To identify open reading frames (ORFs) and potential translation initiation sites.
- To compare TomRSV RNA-2 sequences with related viruses.
Main Methods:
- Nucleotide sequencing of TomRSV RNA-2.
- Bioinformatic analysis to identify ORFs and predict protein sequences.
- Sequence alignment and comparison with other nepoviruses and comoviruses.
Main Results:
- The TomRSV RNA-2 sequence is 7273 nucleotides long (excluding poly(A) tail).
- A single long ORF (5646 nucleotides) was identified, encoding the coat protein in its C-terminal region.
- Significant amino acid similarity was observed between TomRSV and GFLV coat proteins, but limited similarity was found in non-coding regions.
Conclusions:
- The determined sequence provides a comprehensive genetic map of TomRSV RNA-2.
- The findings suggest evolutionary relationships between TomRSV and other nepoviruses, particularly concerning coat protein genes.
- Further research can utilize this sequence data for developing TomRSV resistance strategies.