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Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
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Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis

E Z Ron, R E Kohler, B D Davis

    Science (New York, N.Y.)
    |September 2, 1966
    PubMed

    Abstract:

    Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.

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