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Updated: Aug 13, 2026

10:59
Analysis of Translation Initiation During Stress Conditions by Polysome Profiling
Published on: May 19, 2014
Polysomes extracted from Escherichia coli by freeze-thaw-lysozyme lysis
Abstract:
Polysomes can be extracted from Escherichia coli by freezing and thawing in the presence of lysozyme, followed by treatment with sodium deoxycholate. The method is simple and convenient; the yields consistently high.
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