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Related Experiment Videos

Translocatable elements in Staphylococcus aureus.

R P Novick, I Edelman, P D Latta

    Contributions to Microbiology and Immunology
    |January 1, 1979
    PubMed
    Summary
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    The first described transposable element in Gram-positive bacteria moves between plasmids and chromosomes, conferring erythromycin resistance. This movement can inactivate genes and contributes to plasmid evolution.

    Area of Science:

    • Microbiology
    • Bacterial Genetics
    • Molecular Biology

    Background:

    • Gram-positive bacteria possess mobile genetic elements that contribute to their adaptability.
    • Understanding the mechanisms of DNA translocation is crucial for comprehending bacterial evolution and antibiotic resistance spread.

    Purpose of the Study:

    • To characterize the first identified transposable element in Gram-positive bacteria.
    • To investigate the translocation mechanism and its implications for plasmid evolution.
    • To explore the potential for translocation of other antibiotic resistance genes.

    Main Methods:

    • Description of a 5.2 kb DNA segment from Staphylococcus aureus encoding erythromycin resistance.
    • Analysis of translocation events between plasmid and chromosomal DNA.

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  • Investigation of insertional inactivation and deletion events.
  • Preliminary studies on the translocation of penicillin and chloramphenicol resistance.

    • Main Results:

    • The 5.2 kb element translocates from plasmid to multiple chromosomal sites and vice versa.
    • Translocation can lead to insertional inactivation and deletion of host DNA.
    • Evidence suggests translocation of penicillin and chloramphenicol resistance genes.
    • Translocation of chloramphenicol resistance involves an intact plasmid.

    Conclusions:

    • The characterized transposable element plays a significant role in bacterial genome plasticity.
    • Translocation events contribute to the evolution of bacterial plasmids and the spread of resistance.
    • Further research into these mobile elements is warranted to understand their full impact.