Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Inside-out red cell membrane vesicles: preparation and purification.

T L Steck, R S Weinstein, J H Straus

    Science (New York, N.Y.)
    |April 10, 1970
    PubMed
    Summary

    Researchers created inside-out vesicles from human red blood cell membranes using alkaline buffers. Divalent cations prevented inversion, allowing separation of vesicle types via density gradients.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Issues in modern bone histomorphometry.

    Bone·2011
    Same author

    THE MULTIPLICATION OF BACTERIOPHAGE IN VIVO AND ITS PROTECTIVE EFFECT AGAINST AN EXPERIMENTAL INFECTION WITH SHIGELLA DYSENTERIAE.

    The Journal of experimental medicine·2009
    Same author

    Membrane lipids and the conformations of membrane proteins.

    The Journal of general physiology·2009
    Same author

    Spectroscopic markers of bone quality in alendronate-treated postmenopausal women.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2008
    Same author

    Telemedicine and telepresence for trauma and emergency care management.

    Scandinavian journal of surgery : SJS : official organ for the Finnish Surgical Society and the Scandinavian Surgical Society·2008
    Same author

    Glucocorticoids act directly on osteoclasts to increase their life span and reduce bone density.

    Endocrinology·2006

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Membrane Biophysics

    Background:

    • Human red blood cell membranes are crucial for cellular integrity and function.
    • Understanding membrane vesicle formation is key to studying cellular processes.

    Purpose of the Study:

    • To develop a method for generating inside-out vesicles from human red blood cell membranes.
    • To investigate factors influencing vesicle orientation during preparation.
    • To establish a technique for separating differently oriented vesicles.

    Main Methods:

    • Purification of plasma membranes from human red blood cells.
    • Disruption of membranes in alkaline buffer of low ionic strength to form vesicles.
    • Use of divalent cations to control vesicle orientation.
    • Separation of inside-out and right-side-out vesicles using dextran density gradient centrifugation.

    Main Results:

    • Vesicles were successfully generated from human red blood cell membranes.
    • The majority of vesicles formed were inside-out under specific buffer conditions.
    • Divalent cations significantly inhibited the formation of inside-out vesicles.
    • A density gradient centrifugation method effectively separated inside-out from right-side-out vesicles.

    Conclusions:

    • A reliable method for producing and separating red blood cell membrane vesicles based on orientation was established.
    • Divalent cations play a critical role in regulating the orientation of membrane vesicles during preparation.
    • This technique facilitates further studies on the distinct properties of inside-out and right-side-out vesicles.

    Related Experiment Videos