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Related Experiment Video

Updated: Jul 20, 2026

Fast and Specific Assessment of the Halogenating Peroxidase Activity in Leukocyte-enriched Blood Samples

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Chromate transport in human leukocytes.

D L Lilien, J L Spivak, I D Goldman

The Journal of Clinical Investigation

|August 1, 1970

Summary

This study reveals that chromium transport into human leukocytes is a specific, energy-dependent process. Chromate uptake follows Michaelis-Menten kinetics, indicating a saturable transport system for this essential trace metal.

Area of Science:

Biochemistry
Cell Biology
Trace Element Metabolism

Background:

Chromium is a vital trace metal in human physiology.
51-Chromate is a common radiolabel for studying blood cell kinetics.

Purpose of the Study:

To investigate the transport characteristics of 51-chromate in normal human leukocytes.
To elucidate the kinetics and specificity of chromate uptake by white blood cells.

Main Methods:

Investigated chromate uptake in leukocytes over 1 hour.
Assessed temperature sensitivity (Q10) and metabolic poison effects.
Analyzed influx kinetics using Michaelis-Menten models.
Tested specificity with other divalent anions and metavanadate.

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Last Updated: Jul 20, 2026

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Main Results:

Chromate uptake is unidirectional and nonexchangeable intracellularly.
Influx is temperature-sensitive (Q10 ≈ 2) and inhibited by metabolic poisons, suggesting energy dependence.
Transport follows Michaelis-Menten kinetics with Vmax of 52 mmumoles/g/min and Km of 87 μmoles/liter.
The mechanism is highly specific for chromate, with competitive inhibition by metavanadate.

Conclusions:

Leukocyte chromate transport is a specific, saturable, and energy-dependent process.
Chromate may inhibit cellular energy metabolism, affecting its own uptake.
Understanding this transport is crucial for using 51-chromate in hematological studies.