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Related Experiment Videos

Macro-creatine kinase-BB: observations on 6 patients.

G Chemnitz, E Jockers-Wretou, E Schmidt

    Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
    |November 1, 1979
    PubMed
    Summary

    Six patients exhibited persistent, unexplained high creatine kinase (CK) levels. Immunotitration identified this activity as CK-BB bound to immunoglobulins, a finding missed by other methods.

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    Area of Science:

    • Biochemistry
    • Clinical enzymology
    • Immunology

    Background:

    • Elevated total creatine kinase (CK) activity is a common clinical finding.
    • Persistent and unexplained high CK levels require thorough investigation of isoenzyme patterns.
    • Standard diagnostic methods may misinterpret atypical CK activity.

    Purpose of the Study:

    • To investigate the cause of persistent, inexplicable high creatine kinase activity in six patients.
    • To compare different methods for analyzing serum creatine kinase isoenzymes.
    • To characterize the atypical creatine kinase isoenzyme found in patient sera.

    Main Methods:

    • Analysis of serum isoenzyme patterns using electrophoresis, immunotitration, immunoinhibition, and column chromatography.
    • Characterization of the atypical creatine kinase isoenzyme's molecular weight and substrate affinity.

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  • Investigation of the interaction between creatine kinase-BB and immunoglobulins.

    • Main Results:

    • Electrophoresis showed an atypical band between CK-MM and CK-MB.
    • Immunotitration accurately identified the activity as primarily creatine kinase-BB (CK-BB).
    • Other methods like immunoinhibition and chromatography led to misinterpretations.
    • The atypical CK-BB exhibited higher molecular weight and altered substrate affinity due to immunoglobulin binding.

    Conclusions:

    • Immunotitration is crucial for accurately quantifying persistent, atypical creatine kinase activity.
    • Creatine kinase-BB bound to immunoglobulins can cause misinterpretations with standard analytical methods.
    • This immunoglobulin-CK complex represents a unique form of creatine kinase activity in serum.