Separation of glutathione reductase in human serum by gradient gel electrophoresis

A system of high resolution polyacrylamide gel electrophoresis is introduced. It allows the rapid separation of proteins from serum and tissue homogenates under non-denaturating conditions, and proves especially suited for the comparative analysis of enzymes. The enzyme glutathione reductase is identified on the gradient gels by contact printing with agar substrate gels, and the multiple forms of glutathione reductase in serum are demonstrated.