Haemoglobin A1c separation by micro-column chromatography: a new rapid method of assay

Since at present chromatographic methods for the separation of the glycosylated haemoglobins and determination of haemoglobin A1c are time-consuming and require continuous monitoring, a new micro-column method is reported that is cheap, rapid, easy to perform and permits anything up to 30 columns to be run simultaneously. The haemoglobin A1c leve in fifty normal subjects was measured and found to have a mean of 5.3 +/- 0.5% (m +/- S.D.).