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Related Experiment Videos

Species-specific aggregation factor in sponges. V. Influence on programmed syntheses.

W E Müller, I Müller, R K Zahn

    Biochimica Et Biophysica Acta
    |January 19, 1976
    PubMed
    Summary
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    Sponge cells aggregate and proliferate when exposed to an aggregation factor. This factor stimulates DNA, RNA, and protein synthesis, leading to increased cell division and indicating a transition from a quiescent to a proliferative state.

    Area of Science:

    • Cell Biology
    • Developmental Biology
    • Marine Biology

    Background:

    • Isolated cells and small aggregates of the siliceous sponge Geodia cydonium exhibit limited synthetic and mitotic activity.
    • Cellular communication and aggregation are crucial for multicellular development and tissue formation.

    Purpose of the Study:

    • To investigate the role of an aggregation factor in stimulating cellular proliferation and synthesis in Geodia cydonium.
    • To elucidate the molecular mechanisms underlying aggregation-induced cell cycle progression.

    Main Methods:

    • Primary aggregates of Geodia cydonium cells were cultured and treated with a purified aggregation factor.
    • Rates of DNA, RNA, and protein synthesis were measured.
    • Mitotic activity was assessed by determining the mitotic coefficient.

    Related Experiment Videos

  • Inhibitors of nucleic acid and protein synthesis were used to probe the dependency of aggregation-induced proliferation.

    • Main Results:

    • Addition of the aggregation factor induced the formation of large secondary aggregates (>1000 mum).
    • Secondary aggregation was accompanied by a significant increase in DNA, RNA, and protein synthesis.
    • Mitotic activity dramatically increased, reaching a peak 8 hours post-aggregation.
    • Inhibitors of RNA, protein, and DNA synthesis suppressed the stimulated mitotic activity.

    Conclusions:

    • The aggregation factor triggers a cascade of molecular events leading to cell proliferation in Geodia cydonium.
    • The findings suggest that quiescent cells (G0-phase) enter the cell cycle (G1-phase) in response to aggregation signals.
    • This study provides insights into the regulation of cell proliferation during multicellular development in sponges.