Comparison of surfactant production from kerosene by four species of Corynebacterium

Four species of Corynebacterium-C. fasciens, C. hydrocarboclastus, C. lepus and C. xerosis, were compared for growth and surfactant production from kerosene. Nitrate as sole nitrogen source resulted in a sharp peak in surfactant concentration during mid-exponential growth phase. Ammonium plus nitrate resulted in growth-associated surfactant production. Surfactant was produced at a concentration of 50-80 times the critical micelle concentration by these species; greatest concentrations were produced by C. lepus. All species were pleomorphic, with greatest cell length occurring in early exponential phase. C. hydrocarboclastus had distinctly longer cells (5 mu) than the other species (3 mu) when grown in shake-flask culture. Ammonium sulfate at concentrations greater than 0.1% (w/v) increased cell length in stationary phase.