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Glycosaminoglycans: potential determinants in cardiac morphogenesis
Texas Reports on Biology and Medicine
|January 1, 1979
Summary
6-diazo-5-oxo-L-norleucine (DON) treatment inhibited embryonic heart hyaluronate secretion, delaying AV cushion cell formation and altering cell migration. This supports a link between extracellular glycosaminoglycans and heart cushion development.
Area of Science:
- Developmental biology
- Cardiovascular research
- Extracellular matrix biology
Background:
- Embryonic heart development involves complex cellular processes.
- Atrioventricular (AV) cushion formation is crucial for valvular and septal development.
- Extracellular glycosaminoglycans (GAGs) play roles in tissue morphogenesis.
Purpose of the Study:
- To investigate the role of hyaluronate secretion in early embryonic heart development.
- To determine the impact of inhibiting hyaluronate synthesis on AV cushion cell formation and migration.
- To elucidate the relationship between extracellular GAGs and cardiac cushion morphogenesis.
Main Methods:
- Treatment of embryonic hearts with 6-diazo-5-oxo-L-norleucine (DON) to inhibit hyaluronate secretion.
- Observation of AV cushion cell formation and migration patterns.
- Analysis of sulfated glycosaminoglycan synthesis (chondroitin-6-sulfate and heparan sulfate).
Main Results:
- DON treatment significantly inhibited myocardial hyaluronate secretion.
- AV cushion cell formation was delayed and reduced following DON treatment.
- Sulfated GAG synthesis was affected later, coinciding with altered cushion cell migration.
- Results indicate a causal link between extracellular GAGs and cardiac cushion tissue morphogenesis.
Conclusions:
- Hyaluronate secretion by the myocardium is critical for timely AV cushion cell formation.
- Extracellular GAGs, particularly hyaluronate, are essential for normal cardiac cushion development and cell behavior.
- This study provides evidence for a direct role of GAGs in the morphogenesis of heart cushion tissue.