Model of red blood cell rotation in the flow toward a cell sizing orifice. Application to volume distribution

The rotation of human red blood cells (RBC) as they flow in the shear field established by a Coulter type orifice is modeled. This model, based on hydrodynamics of ellipsoid rotation in laminar creeping flow, is used to calculate the probability of the cells entering the orifice with a specific orientation. The electrical resistance change produced by a cell passing through the orifice of an electronic cell volume detector is the product of an orientation-dependent shape factor and the cell volume. This paper presents a method to calculate the shape factor probability distribution which can be used to predict its effect on the cell volume distribution. Experimental results confirm the theoretical prediction that the right skewness of resistance change distributions is in part a result of the nonspherical shape of red cells.