The subcellular distribution of platelet lipids labeled by acetate-1-14C

The lipids of intact human platelets were labeled in vitro with acetate-1-(14)C, and the distribution of radioactivity in individual fatty acids and in lipid classes was examined in platelet subcellular fractions separated by sucrose density gradient ultracentrifugation. The distribution of newly formed fatty acids among individual lipid classes was similar in all subcellular components, and no highly unusual or characteristic lipid metabolic pool was present in either the soluble, membrane, or granule fractions.