Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ferritin in the fungus Phycomyces.

C N David, K Easterbrook

    The Journal of Cell Biology
    |January 1, 1971
    PubMed
    Summary

    This study purified iron-protein ferritin from the fungus Phycomyces blakesleeanus, revealing its structure and location within the organism. Ferritin iron levels are shown to be regulated by external iron availability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    High-resolution characterization of short-term temporal variability in the taxonomic and resistome composition of wastewater influent.

    Microbial genomics·2023
    Same author

    Overweight and Abdominal Obesity Association with All-Cause and Cardiovascular Mortality in the Elderly Aged 80 and Over: A Cohort Study.

    The journal of nutrition, health & aging·2017
    Same author

    Quantitative analysis of cell types during growth and morphogenesis in Hydra.

    Wilhelm Roux' Archiv fur Entwicklungsmechanik der Organismen·2017
    Same author

    Oogenesis in Hydra: nurse cells transfer cytoplasm directly to the growing oocyte.

    Developmental biology·2005
    Same author

    A switch in disulfide linkage during minicollagen assembly in Hydra nematocysts.

    The EMBO journal·2001
    Same author

    Identification of caspases and apoptosis in the simple metazoan Hydra.

    Current biology : CB·1999

    Area of Science:

    • Biochemistry
    • Mycology
    • Cell Biology

    Background:

    • Ferritin is a ubiquitous iron-storage protein found in various organisms.
    • Understanding ferritin's role in fungi like Phycomyces blakesleeanus is crucial for comprehending iron homeostasis in eukaryotic microbes.

    Purpose of the Study:

    • To purify and characterize ferritin from Phycomyces blakesleeanus.
    • To investigate the subcellular localization and iron-dependent regulation of ferritin in this fungus.

    Main Methods:

    • Purification of ferritin from fungal mycelium, sporangiophores, and spores.
    • Biophysical characterization including sedimentation coefficient, buoyant density, and electron microscopy.
    • Analysis of ferritin localization via electron microscopy of thin-sections.
    • Iron-supplementation experiments to assess regulatory mechanisms.

    Main Results:

    • Purified Phycomyces ferritin exhibits characteristic properties (protein-to-iron ratio 5, 55S sedimentation coefficient, 1.82 g/cm(3) buoyant density).
    • Apoferritin shows an 18S sedimentation coefficient, with subunits of 25,000 molecular weight.
    • Ferritin forms crystalline monolayers on lipid droplets in the cytoplasm, particularly abundant in spores.
    • Ferritin iron content increases up to 50-fold in response to iron-supplemented growth media.

    Conclusions:

    • Phycomyces blakesleeanus possesses a well-defined ferritin system for iron storage.
    • Ferritin localization on lipid droplets suggests a specific functional role within the cell.
    • The iron-storage capacity of ferritin is dynamically regulated by extracellular iron concentrations.

    Related Experiment Videos