[Quantitative evaluation methods of the chemoantibiotic associations (author's transl)]

Area of Science:

• Pharmacology and Microbiology
• Drug Interaction Analysis
• Quantitative Biology

Background:

• Existing classifications of joint drug actions are limited.
• Current bacteriological and biometrical techniques for evaluating chemoantibiotic interactions have limitations.
• The isobolic model for interaction evaluation faces criticism.

Purpose of the Study:

• To propose a novel classification of joint drug actions, including synergism subtypes.
• To classify and critically analyze existing bacteriological and biometrical techniques for assessing chemoantibiotic interactions.
• To introduce a new quantitative method, the 'isoeffectual' procedure, for evaluating drug interactions.

Main Methods:

• A new classification system for joint drug actions (interference, cooperation, interaction) is presented.
• Bacteriological techniques (in vitro and in vivo) for evaluating chemoantibiotic interactions are classified.
• A novel 'isoeffectual' procedure involving a grid of drug concentrations and planar diagram analysis is proposed.

Main Results:

• The study classifies interactions into interference, cooperation, and true interaction (with synergism subtypes).
• It critically analyzes existing methods for evaluating drug interactions, highlighting limitations of the isobolic model.
• The proposed 'isoeffectual' procedure generates curvilinear regressions explained by a mathematical formula for quantitative analysis.

Conclusions:

• The proposed classification provides a framework for understanding joint drug actions.
• The 'isoeffectual' procedure offers a robust method for quantitative evaluation of chemoantibiotic interactions.
• This approach establishes a generalizable mathematical model for analyzing combined drug effects.