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Histidase activity in cultivated human amniotic fluid cells
Science (New York, N.Y.)
|August 13, 1971
Summary
Amniotic fluid cell cultures contain distinct cell types. Epithelial cells show high histidase activity, unlike fibroblasts from the same culture, revealing biochemical differences.
Area of Science:
- Biochemistry
- Cell Biology
- Genetics
Background:
- Amniotic fluid cell cultures are used for prenatal diagnosis and research.
- Understanding cell heterogeneity within these cultures is crucial for accurate interpretation.
Purpose of the Study:
- To investigate the biochemical properties of different cell types within amniotic fluid cell cultures.
- To determine if distinct cell populations exhibit differential enzyme activity.
Main Methods:
- Isolation and culture of epithelial and fibroblast cells from amniotic fluid.
- Assay of histidase enzyme activity in both cell types.
Main Results:
- Epithelial cells derived from amniotic fluid cultures exhibited high histidase activity.
- Fibroblast cells from the same cultures showed no detectable histidase activity.
Conclusions:
- Amniotic fluid cell cultures are composed of heterogeneous cell populations.
- Differential enzyme expression, such as histidase, distinguishes cell types within these cultures.