Related Concept Videos

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model07:34

Quantification of Circulating Pig-Specific DNA in the Blood of a Xenotransplantation Model

In this protocol, porcine specific primers were designed, plasmids-containing porcine specific DNA fragments were constructed, and standard curves for quantitation were established. Using species-specific primers, cpsDNA was quantified by qPCR in pig-to-mouse cell transplantation models and pig-to-monkey artery patch transplantation...
4.4K
Capture and Release of Viable Circulating Tumor Cells from Blood08:10

Capture and Release of Viable Circulating Tumor Cells from Blood

A protocol to utilize a poly(N-iso-propylacrylamide) (PIPAAm) coated microfilter for effective capture and thermoresponsive release of viable circulating tumor cells (CTC) is presented. This method allows capture of CTC from patients' blood and subsequent release of viable CTC for downstream off-chip culture, analyses and...
9.0K
Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells08:03

Generation of Prostate Cancer Patient Derived Xenograft Models from Circulating Tumor Cells

This manuscript details a method used to generate prostate cancer patient derived xenografts (PDXs) from circulating tumor cells (CTCs). The generation of PDX models from CTCs provides an alternative experimental model to study prostate cancer; the most commonly diagnosed tumor and a frequent cause of death from cancer in...
14.6K
Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples07:32

Rapid Isolation of Viable Circulating Tumor Cells from Patient Blood Samples

Circulating tumor cells are isolated from the blood of cancer patients without inflicting cellular damage. Isolation of tumor cells is accomplished using a bimolecular surface of E-selectin in addition to antibodies against epithelial markers. A nanotube coating specifically promotes cancer cell adhesion resulting in high capture...
27.2K
Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells09:53

Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells

An automated microfluidic device was developed for circulating nucleated cell enrichment from peripheral blood via erythrocyte lysis that ensures isolation of high quality sample without cell...
12.7K
Vapor Pressure Lowering03:28

Vapor Pressure Lowering

The equilibrium vapor pressure of a liquid is the pressure exerted by its gaseous phase when vaporization and condensation are occurring at equal rates:
30.7K