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Comparison of rabbit and pig plasma in the tube coagulase test
Abstract:
A total of 627 clinical isolates of Micrococcaceae were characterized accoridng to their ability to coagulate rabbit and pig plasma, produce thermostable nuclease, and anaerobically ferment glucose and mannitol. By using these characteristics, 416 of the isolates were classified as Staphylococcus aureus and 211 as non-S. aureus. All 416 strains produced a 3 to 4+ clot formation in heparinized pig plasma, whereas 415 isolates produced a similar reaction in citrated rabbit plasma. All of the S. aureus strains possessed a thermostable nuclease, whereas only four of the non-S. aureus isolates exhibited this characteristic. The results obtained using heparinized pig plasma were almost identical to those obtained with commercial rabbit plasma.
Insights
This study classified 627 Micrococcaceae isolates, identifying 416 as Staphylococcus aureus. Key tests included plasma coagulation and thermostable nuclease production for accurate bacterial identification.
Area of Science:
- Microbiology
- Clinical Diagnostics
Background:
- Accurate identification of bacterial pathogens is crucial in clinical settings.
- Staphylococcus aureus is a significant human pathogen requiring reliable diagnostic methods.
Purpose of the Study:
- To evaluate the efficacy of plasma coagulation and thermostable nuclease production for differentiating Staphylococcus aureus from other Micrococcaceae.
- To compare the diagnostic performance of pig plasma and rabbit plasma.
Main Methods:
- Characterization of 627 clinical Micrococcaceae isolates.
- Assays included rabbit and pig plasma coagulation, thermostable nuclease production, and anaerobic glucose/mannitol fermentation.
- Isolates were classified as Staphylococcus aureus or non-S. aureus based on these tests.
Main Results:
- 416 isolates were identified as Staphylococcus aureus and 211 as non-S. aureus.
- All Staphylococcus aureus strains showed positive results for plasma coagulation and thermostable nuclease production.
- Results from heparinized pig plasma closely matched those from citrated rabbit plasma.
Conclusions:
- Plasma coagulation and thermostable nuclease assays are effective for identifying Staphylococcus aureus.
- Heparinized pig plasma offers a reliable alternative to commercial rabbit plasma for these diagnostic tests.