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Updated: Aug 4, 2026

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RNA In situ Hybridization in Whole Mount Embryos and Cell Histology Adapted for Marine Elasmobranchs
Published on: April 12, 2013
The RNA of unfertilized sea urchin eggs is "capped"
Abstract:
It is shown that the RNA of unfertilized sea urchin eggs is active in stimulating protein synthesis in a wheat germ cell free system. This activity is not lowered by conditions that inhibit the methylation processes but is inhibited by a treatment that damages the "cap", A difference in activity in a wheat germ cell free system between the RNA of unfertilized eggs and the RNA from early embryos is described.
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps the cell...

