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Antibodies specific to two deoxyribotrinucleotide sequences.

S A Khan, T M Jacob

    Nucleic Acids Research
    |September 1, 1977
    PubMed
    Summary
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    Researchers developed specific antibodies for deoxyribotrinucleotides dpApTpA and dpApApT. These antibodies precisely recognize their target DNA sequences, paving the way for advanced molecular diagnostics.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Biochemistry

    Background:

    • Deoxyribotrinucleotides are fundamental building blocks of DNA.
    • Developing specific antibodies for short DNA sequences is crucial for molecular detection and research.

    Purpose of the Study:

    • To generate and characterize antibodies against specific deoxyribotrinucleotide sequences, namely dpApTpA and dpApApT.
    • To assess the specificity of these antibodies for their target sequences.

    Main Methods:

    • Antibodies were prepared by immunizing rabbits with bovine serum albumin conjugates of dpApTpA and dpApApT haptens.
    • Antibody specificity was determined using a nitrocellulose membrane binding assay.
    • The assay measured the inhibition of tritiated hapten binding by various nonradioactive oligonucleotides.

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    Main Results:

    • Successfully generated antisera containing antibodies against dpApTpA and dpApApT.
    • The anti-dpApTpA and anti-dpApApT antibodies demonstrated high specificity for their respective hapten sequences.
    • The binding assay confirmed the precise recognition of target sequences by the developed antibodies.

    Conclusions:

    • The study successfully produced highly specific antibodies for deoxyribotrinucleotides dpApTpA and dpApApT.
    • These specific antibodies can be valuable tools in molecular biology and diagnostics for detecting specific DNA sequences.