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Experimental infection of white mouse with Chrysosporium and Paecilomyces
Abstract:
SPF white mice were inoculated intraperitoneally with 5 strains of saprophytic fungi of the mycelial genera Chrysosporium (C. keratinophilum, C. tropicum) and Paecilomyces (P. lilacinus, P. marquandii, P. victoriae). The fungi caused granulomatous lesions in the peritoneal cavity and they were recultured (except P. lilacinus and P. marquandii) two months after inoculation. Spores, short hyphae and budding cells of all the fungi were observed in the granulomas stained by periodic-acid Schiff (PAS) and methenamine-silver nitrate (Grocott) techniques.
Insights
Saprophytic fungi, including Chrysosporium and Paecilomyces, caused granulomatous lesions in mice. Fungal elements were observed in these lesions two months post-inoculation, indicating a persistent infection.
Area of Science:
- Mycology
- Immunology
- Infectious Diseases
Background:
- Saprophytic fungi are commonly found in the environment.
- Some fungi can cause opportunistic infections in susceptible hosts.
- Understanding fungal pathogenesis is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the pathogenicity of five saprophytic fungal strains in a murine model.
- To characterize the resulting lesions and identify fungal elements within them.
Main Methods:
- SPF white mice were inoculated intraperitoneally with five strains of Chrysosporium and Paecilomyces.
- Granulomatous lesions in the peritoneal cavity were analyzed.
- Fungal elements were identified using periodic-acid Schiff (PAS) and Grocott (methenamine-silver nitrate) staining techniques.
Main Results:
- All five fungal strains induced granulomatous lesions in the peritoneal cavity.
- Fungi were successfully recultured from lesions in most mice after two months (except P. lilacinus and P. marquandii).
- Spores, hyphae, and budding cells of the fungi were visualized within the granulomas.
Conclusions:
- Chrysosporium and Paecilomyces species can induce persistent granulomatous lesions in mice.
- These findings highlight the potential pathogenicity of these saprophytic fungi.
- Further research is warranted to explore the clinical significance and treatment strategies for such infections.