Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

In vitro interaction of L1210 cells with phospholipid vesicles.

V K Jansons, P Weis, T Chen

    Cancer Research
    |March 1, 1978
    PubMed
    Summary

    Liposomes, or phospholipid vesicles, are primarily absorbed by leukemia cells through an energy-dependent process. Positively charged liposomes show significantly higher cellular uptake compared to neutral or negatively charged ones.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Dismal prognosis of Pneumocystis jirovecii pneumonia in patients with multiple myeloma.

    Annals of hematology·2023
    Same author

    Quantification of A. fumigatus-specific CD154+ T-cells-preanalytic considerations.

    Medical mycology·2016
    Same author

    Towards loss compensated and lasing terahertz metamaterials based on optically pumped graphene.

    Optics express·2014
    Same author

    [Correlation of patients age on length of stay and admission rate in a German emergency department].

    Deutsche medizinische Wochenschrift (1946)·2013
    Same author

    Porphyry-copper ore shells form at stable pressure-temperature fronts within dynamic fluid plumes.

    Science (New York, N.Y.)·2012
    Same author

    PCDD/F concentrations of agricultural soil in the vicinity of fluidized bed incinerators of co-firing MSW with coal in Hangzhou, China.

    Journal of hazardous materials·2007

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Nanotechnology

    Background:

    • Phospholipid vesicles, or liposomes, are crucial in drug delivery and biological studies.
    • Understanding liposome-cell interactions is key to optimizing their therapeutic applications.
    • Leukemia L1210 cells provide a model system for investigating cellular uptake mechanisms.

    Purpose of the Study:

    • To investigate the in vitro uptake mechanisms of phospholipid vesicles by mouse leukemia L1210 cells.
    • To determine the influence of liposome charge and cell culture conditions on uptake efficiency.
    • To elucidate the energy-dependent nature and surface interactions involved in liposome internalization.

    Main Methods:

    • Liposomes were prepared using ultrasonic dispersion and characterized by electron microscopy.
    • Cellular uptake was quantified by measuring cell-associated radioactivity.
    • Temperature dependence, metabolic inhibitors, and cell culture age were assessed.
    • The effect of liposome surface charge (neutral, negative, positive) was evaluated.
    • Cell surface treatments (trypsin, neuraminidase, NaCl) were used to probe interaction sites.

    Main Results:

    • Liposome uptake was temperature-dependent and inhibited by metabolic inhibitors, indicating an energy-dependent process.
    • Uptake was approximately twofold higher in young cell cultures compared to stationary cultures.
    • Positively charged liposomes exhibited a ~20-fold greater uptake than neutral or negatively charged vesicles.
    • A significant portion of the radioactivity from positively charged liposomes could be removed by enzymatic or ionic treatments.

    Conclusions:

    • Liposome internalization by L1210 cells is predominantly an active, energy-requiring process.
    • Liposome surface charge, particularly positive charge, significantly enhances cellular uptake.
    • Cell surface components likely play a role in the interaction and uptake of charged liposomes.

    Related Experiment Videos