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Infection of cultivated mouse peritoneal macrophages with lymphocytic choriomeningitis virus
Abstract:
Lymphocytic choriomeningitis virus strain WE multiplied in cultivated peritoneal macrophages from unstimulated NMRI mice. As revealed by immunofluorescence technique, most cells participated in the infectious process. They were not, however, functionally altered. Morphologically, infected cells contained more cytoplasmic vacuoles, but these were also present, though less numerous, in uninfected ageing cells and in cells having phagocytosed Latex beads. As compared with the WE strain virus, the E-350 (Armstrong) strain multiplied more slowly to lower titres, but the proportion of infected cells was similar.
Insights
Lymphocytic choriomeningitis virus (LCMV) strain WE infects most peritoneal macrophages in NMRI mice without functional alteration. Another LCMV strain, E-350, showed slower multiplication and lower viral titers.
Area of Science:
- Virology
- Immunology
- Cell Biology
Background:
- Lymphocytic choriomeningitis virus (LCMV) is an important pathogen.
- Understanding LCMV interactions with host cells is crucial for disease control.
Purpose of the Study:
- To investigate the interaction between LCMV strain WE and peritoneal macrophages from NMRI mice.
- To compare the infectivity and replication of LCMV strain WE and E-350 in these cells.
Main Methods:
- Cultivation of peritoneal macrophages from NMRI mice.
- Infection with LCMV strains WE and E-350.
- Immunofluorescence technique to detect infected cells.
- Morphological analysis of infected and uninfected cells.
Main Results:
- LCMV strain WE efficiently infected most peritoneal macrophages.
- Infected cells showed increased cytoplasmic vacuoles but no functional alterations.
- LCMV strain E-350 replicated slower and to lower titers compared to strain WE.
- The proportion of infected cells was similar for both strains.
Conclusions:
- LCMV strain WE infects peritoneal macrophages without causing significant functional impairment.
- Differences in replication kinetics exist between LCMV strains WE and E-350.
- Cytoplasmic vacuole formation is a morphological change associated with LCMV infection and aging cells.