Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endogenous estradiol-17beta in bovine tissues.

D M Henricks, A K Torrence

    Journal - Association of Official Analytical Chemists
    |September 1, 1978
    PubMed
    Summary

    A new radioimmunoassay accurately measures estradiol-17beta in bovine tissues. Estradiol-17beta concentrations were highest in uterine endometrium, followed by liver and kidney, with variations noted between sexes and estrous cycle phases.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Measurement of leptin and insulin-like growth factor-I in seminal plasma from different species.

    Physiological research·2002
    Same author

    Residues from anabolic preparations after good veterinary practice.

    APMIS : acta pathologica, microbiologica, et immunologica Scandinavica·2001
    Same author

    Crosstalk and considerations in endocrine disruptor research.

    Medical hypotheses·2001
    Same author

    Actions and interactions of the IGF system in Alzheimer's disease: review and hypotheses.

    Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society·2001
    Same author

    Synergistic approach to cancer therapy: exploiting interactions between anti-estrogens, retinoids, monoterpenes and tyrosine kinase inhibitors.

    Medical hypotheses·2000
    Same author

    Physiological basis for use of insulin-like growth factors in reproductive applications: a review.

    Theriogenology·2000

    Area of Science:

    • Veterinary Endocrinology
    • Analytical Chemistry
    • Food Science

    Background:

    • Estradiol-17beta (E2beta) is a key estrogen influencing reproductive cycles and tissue development in cattle.
    • Understanding E2beta distribution in edible tissues is crucial for food safety and reproductive studies.
    • Accurate quantification methods are needed to assess E2beta levels in various bovine tissues.

    Purpose of the Study:

    • To develop and validate a radioimmunoassay (RIA) for quantifying estradiol-17beta in bovine muscle, kidney, liver, and uterine endometrium.
    • To determine the accuracy, reproducibility, and sensitivity of the developed RIA method.
    • To compare E2beta concentrations across different bovine tissues and investigate variations related to sex and estrous cycle phase.

    Main Methods:

    • Bovine tissues (muscle, kidney, liver, uterine endometrium) were homogenized, extracted, and partitioned.
    • Radioimmunoassay (RIA) was employed for E2beta quantification; chromatography was not utilized.
    • Assay validation included assessment of accuracy, reproducibility, and sensitivity.

    Main Results:

    • The RIA demonstrated good accuracy, reproducibility, and sensitivity for E2beta measurement.
    • E2beta concentrations were highest in uterine endometrium (10-fold higher than muscle), followed by liver and kidney (3-fold higher than muscle).
    • Significant differences in E2beta levels were observed in the liver and kidney between heifers and steers, and in the endometrium between different estrous cycle phases in heifers.

    Conclusions:

    • A reliable RIA method for measuring E2beta in bovine tissues has been established.
    • Tissue-specific distribution of E2beta varies significantly, with the reproductive tract showing the highest concentrations.
    • Sex and reproductive status influence E2beta levels in certain bovine tissues, providing valuable insights for endocrine and food safety research.

    Related Experiment Videos