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Related Experiment Videos

Electrical coupling and uncoupling of exocrine acinar cells.

N Iwatsuki, O H Petersen

    The Journal of Cell Biology
    |November 1, 1978
    PubMed
    Summary

    Mouse pancreatic acinar cells are electrically coupled, but acetylcholine can reversibly uncouple them. This uncoupling reduces cell membrane surface area and occurs at low stimulant concentrations.

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    Cell calcium·2011

    Area of Science:

    • Cellular electrophysiology
    • Gastrointestinal physiology
    • Intercellular communication

    Background:

    • Pancreatic acinar cells form an electrical communication network crucial for tissue function.
    • Understanding cell-to-cell coupling is key to deciphering coordinated acinar responses.

    Purpose of the Study:

    • To investigate the electrical coupling characteristics within mouse pancreatic acinar tissue.
    • To determine the effects of acetylcholine (ACh) and other stimulants on this electrical coupling.

    Main Methods:

    • Simultaneous intracellular microelectrode recordings in mouse pancreatic acinar tissue.
    • Microiontophoresis of acetylcholine (ACh) to assess uncoupling effects.
    • Microscopical localization of microelectrode tips.

    Main Results:

    • Cells within a single acinus exhibit strong electrical coupling (coupling coefficient near 1).
    • Electrical coupling decreases with distance and is rarely observed beyond 110 microns.
    • Acetylcholine (ACh) application rapidly and reversibly uncouples acinar cells, reducing electrical time constant and input capacitance.
    • Low concentrations of ACh, caerulein, and bombesin induce uncoupling, lower than those for maximal enzyme secretion.

    Conclusions:

    • Mouse pancreatic acinar cells are extensively electrically coupled, forming a syncytium.
    • Acetylcholine acts as a potent regulator of intercellular electrical communication in pancreatic acinar tissue.
    • ACh-induced uncoupling may represent a mechanism to modulate acinar cell responsiveness and function.

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