Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Procedural pain management in pediatric oncology: a review of the literature.

Cancer investigation·1999
Same author

Empirically supported treatments in pediatric psychology: disease-related pain.

Journal of pediatric psychology·1999
Same author

A fluorescent probe for the active site of bovine trypsin.

Physiological chemistry and physics and medical NMR·1983
Same author

A new chromogenic substrate for angiotensin-converting enzyme.

Analytical biochemistry·1978
Same author

The activity of trypsin.

Journal of chemical education·1977
Same author

Fluorescent probe studies of haptoglobin type 2-1.

Physiological chemistry and physics·1976

Related Experiment Video

Updated: Jul 24, 2026

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
08:50

Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

Published on: October 10, 2013

A fluorescent probe study of salmine AI.

S F Russo, R G Engel

    Physiological Chemistry and Physics
    |January 1, 1978
    PubMed
    Summary

    This study used the 1-p-toluidinylnapthalene-8-sulfonate (1,8-TNS) fluorescent probe to identify moderately nonpolar binding sites on salmine AI. Findings reveal cooperative binding influenced by ionic strength and acidity.

    Area of Science:

    • Biochemistry
    • Biophysical Chemistry

    Background:

    • Salmine AI is a protamine, a small, arginine-rich protein.
    • Understanding protein-ligand interactions is crucial in molecular biology and drug discovery.

    Purpose of the Study:

    • To investigate the nonpolar binding sites on salmine AI using a fluorescent probe.
    • To characterize the binding interactions between salmine AI and 1,8-TNS.

    Main Methods:

    • Fluorescence spectroscopy utilizing 1-p-toluidinylnapthalene-8-sulfonate (1,8-TNS) as a probe.
    • Equilibrium dialysis and thin film dialysis to assess binding kinetics and affinity.

    Main Results:

    • 1,8-TNS exhibited fluorescence enhancement at 497-500 nm upon binding to salmine AI, indicating moderately nonpolar sites.

    More Related Videos

    In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
    09:56

    In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

    Published on: August 18, 2017

    Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
    07:10

    Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

    Published on: July 8, 2025

    Related Experiment Videos

    Last Updated: Jul 24, 2026

    Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
    08:50

    Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles

    Published on: October 10, 2013

    In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS
    09:56

    In Situ Characterization of Shewanella oneidensis MR1 Biofilms by SALVI and ToF-SIMS

    Published on: August 18, 2017

    Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
    07:10

    Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

    Published on: July 8, 2025

  • Binding affinity decreased with increasing ionic strength (0.002 M to 0.050 M).
  • Positive cooperativity was observed in the binding of 1,8-TNS to salmine AI.

    • Conclusions:

    • Salmine AI possesses moderately nonpolar binding sites accessible to 1,8-TNS.
    • The binding interaction is sensitive to environmental factors like ionic strength and acidity.
    • Thin film dialysis offers a more rapid and stable method for studying probe-protein interactions compared to equilibrium dialysis.