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Evaluation of the fibrinogen analyzer.
The American Journal of Medical Technology
|December 1, 1978
Summary
A new fibrinogen analyzer offers reliable plasma fibrinogen assays. It shows high reproducibility and minimal interference from heparin, hemoglobin, and bilirubin, making it ideal for clinical labs.
Area of Science:
- Clinical chemistry
- Hematology
Background:
- Accurate plasma fibrinogen measurement is crucial for diagnosing and managing coagulation disorders.
- Existing methods for fibrinogen assay may have limitations in terms of speed, reproducibility, or interference.
Purpose of the Study:
- To evaluate the clinical utility and performance of a newly introduced automated fibrinogen analyzer.
- To assess the accuracy, reproducibility, and potential interferences of the new instrument for plasma fibrinogen determination.
Main Methods:
- The fibrinogen analyzer was evaluated using automated measurement of absorbance changes during thrombin-induced fibrinogen clotting.
- Assay performance was assessed by comparing results with the established phenol method.
- Interference studies were conducted using varying concentrations of heparin, free hemoglobin, and bilirubin.
Main Results:
- The fibrinogen analyzer demonstrated high reproducibility in plasma fibrinogen assays.
- Results from the analyzer showed a close correlation with the conventional phenol method.
- Heparin concentrations below 1.25 μg/mL, free hemoglobin below 400 mg/dL, and bilirubin below 4 mg/dL had minimal or no significant effect on fibrinogen values.
Conclusions:
- The new fibrinogen analyzer is a reliable and accurate instrument for plasma fibrinogen determination.
- Its robustness against common interfering substances makes it suitable for routine clinical laboratory use.
- The instrument is highly recommended for clinical laboratories performing plasma fibrinogen assays.