[Multiple forms of pectin trans-eliminase from Verticillium dahliae Klebahn -- cotton wilt agent]

From the culture liquid filtrate of Verticillium dahliae--cotton wilt agent--pectin trans-eliminase (EC) was isolated. The enzyme was isolated and examined, using ultrafiltration, gel filtration, ion exchange chromatography, isoelectrofocusing, and electrophoresis. The fungus was found capable to produce several forms of pectin trans-eliminase that differed in their molecular weight, charge, synthesis and release regulation, substrate action (position of bonding breakdowns in the pectin polymer molecule). Pectin trans-eliminase activity was also detected in cell walls of the fungal mycelium. Possible origin of multiple forms of the enzyme is discussed.