Failure of deodorized males to induce oestrus in the wild mouse

The ability of female mice to return to oestrus following exposure to males perfumed either with oil of wintergreen or with the commercial perfume, "Kanta" was evaluated. Unisexual grouping of female mice induced anoestrus in all individuals. Oestrus was, however, promptly induced in the majority of unisexually grouped females by exposure to normal males. By contrast, exposure to perfumed males failed to induce oestrus in unisexually grouped females. The results suggest that male urine which is the source of the primer pheromone involved in the induction of oestrus was ineffective because of the masking effect of artificial scents. Hence the unisexually grouped females were unable to perceive the pheromone from males and continued to remain in anoestrus following exposure to perfumed males. The results provide additional evidence in support of the view that the urinary pheromone produced by males induces oestrus in females by acting through olfactory pathways.