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Immunoassay of human TSH using dried blood samples
Summary
A new radioimmunoassay method can detect elevated thyroid-stimulating hormone (TSH) concentrations in dried blood spots. This sensitive assay is suitable for newborn screening programs to identify congenital hypothyroidism.
Area of Science:
- Endocrinology
- Neonatal screening
- Immunoassay development
Background:
- Congenital hypothyroidism (CH) requires early detection for proper management.
- Existing screening methods may need further refinement for sensitivity and efficiency.
Purpose of the Study:
- To describe a sensitive, semi-quantitative radioimmunoassay (RIA) for screening elevated TSH concentrations.
- To evaluate the method's suitability for newborn screening programs.
Main Methods:
- Utilized dried blood spots (2 x 0.32 cm dots) on filter paper.
- Employed a 3-day incubation period with polyethylene glycol for separation.
- Used a highly sensitive antiserum for detection.
Main Results:
- The method can detect TSH concentrations as low as 22 mμU/ml.
- Elevated TSH levels were observed in dried cord and newborn blood from infants with CH.
- The assay demonstrated sufficient sensitivity for screening purposes.
Conclusions:
- The described RIA method is a sensitive tool for screening elevated TSH.
- It is well-suited for newborn screening programs to identify potential cases of primary hypothyroidism.