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Purification of C-reactive protein on DEAE-cellulose by a simple two-step procedure utilizing the calcium-dependency
Biochimica Et Biophysica Acta
|December 20, 1977
Abstract:
A simple procedure for isolating C-reactive protein is presented. The method consists of two chromatographic separations on diethylaminoethyl (DEAE)-cellulose columns and utilized the difference between the binding of C-reactive protein to DEAE in the presence and absence of calcium. Electrophoretically pure C-reactive protein can be recovered from serum or ascitic fluid with a yield of approx. 50--60% within one day.
