Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Delayed chloroplast luminescence. Activation and suppression].

N i Chan Van, V V Nikandrov, A A Krasnovkiĭ

    Biofizika
    |November 1, 1977
    PubMed
    Summary

    Diethyl ether and Triton X-100 inhibit delayed luminescence (DL) in chloroplasts, while heating, glycerine, and sucrose activate it. These agents affect electron transport and chloroplast membranes differently.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    [Role of the heterotrophic bacteria associated with the cyanobacterium Nostoc muscorum in cadmium sulfide synthesis].

    Mikrobiologiia·2003
    Same author

    Enzymatic oxidation of cadmium and lead metals photodeposited on cadmium sulfide.

    Bioelectrochemistry (Amsterdam, Netherlands)·2001
    Same author

    Inorganic semiconductors as photosensitizers in biochemical redox reactions.

    Membrane & cell biology·1999
    Same author

    Light induced redox reactions involving mammalian ferritin as photocatalyst.

    Journal of photochemistry and photobiology. B, Biology·1998
    Same author

    Metal as a novel type of the enzyme substrate. Metallic cadmium photogenerated in the system CdS-formate as a substrate of the NAD-dependent hydrogenase.

    FEBS letters·1993
    Same author

    Photogeneration of NADH under coupled action of CdS semiconductor and hydrogenase from Alcaligenes eutrophus without exogenous mediators.

    FEBS letters·1992

    Area of Science:

    • Plant Physiology
    • Chloroplast Biology
    • Biophysics

    Background:

    • Delayed luminescence (DL) in chloroplasts is a sensitive indicator of photosynthetic electron transport and energy transduction.
    • Understanding factors affecting DL is crucial for elucidating chloroplast membrane dynamics and function.

    Purpose of the Study:

    • To investigate the impact of specific chemical agents and physical treatments on chloroplast delayed luminescence.
    • To differentiate the mechanisms by which these agents influence electron transport and DL.

    Main Methods:

    • Chloroplasts were treated with diethyl ether, Triton X-100, glycerine, sucrose, and subjected to preliminary heating.
    • Delayed luminescence measurements were performed to assess the effects of these treatments.
    • Electron transport activity was monitored concurrently.

    Main Results:

    • Diethyl ether and Triton X-100, at concentrations that stimulate electron transport, inhibited DL, mimicking photophosphorylation uncouplers.
    • Preliminary heating (42-42°C), glycerine, and sucrose treatments activated both electron transport and DL.
    • The observed activation under heating, glycerine, and sucrose was not attributed to photophosphorylation uncoupling.

    Conclusions:

    • Diethyl ether and Triton X-100 likely interfere with chloroplast energy coupling processes.
    • Heating, glycerine, and sucrose may enhance DL and electron transport by inducing conformational changes in chloroplast membranes.
    • These findings suggest distinct mechanisms underlying the modulation of chloroplast function by different external agents.

    Related Experiment Videos