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Susceptibility to two strains of Friend leukemia virus in mice
Abstract:
A mutant strain of the Friend leukemia virus is described. The parental virus strain, passaged through ICR/Ha mice, shows no or very little activity by the spleen-focus assay in BALB/c mice (by comparison with highly susceptible DBA/2 and ICR/Ha mice) and produces typical Friend disease in these mice only exceptionally; susceptibility to this strain of virus is recessive in the (C57BL/6 x DBA/2) F(1). By contrast, the mutant virus strain is as active in BALB/c mice as in DBA/2 or ICR/Ha mice; susceptibility to the mutant virus strain is dominant in the (C57BL/6 x DBA/2) F(1).
Insights
A newly identified mutant Friend leukemia virus strain exhibits high activity in BALB/c mice, unlike its parental strain. Susceptibility to this mutant virus demonstrates dominant inheritance in (C57BL/6 x DBA/2) F(1) mice.
Area of Science:
- Virology
- Immunology
- Genetics
Background:
- The Friend leukemia virus (FLV) complex causes spleen and hematopoietic disorders.
- FLV exhibits varying pathogenicity and host susceptibility across different mouse strains.
- Genetic factors significantly influence host responses to FLV infection.
Purpose of the Study:
- To characterize a novel mutant strain of FLV.
- To investigate the genetic basis of susceptibility to FLV in different mouse models.
- To compare the pathogenicity of the parental FLV strain with its mutant counterpart.
Main Methods:
- Passaging of the parental FLV strain through ICR/Ha mice.
- Spleen-focus assay in susceptible (DBA/2, ICR/Ha) and less susceptible (BALB/c) mouse strains.
- Genetic crosses using (C57BL/6 x DBA/2) F(1) mice to determine inheritance patterns of susceptibility.
Main Results:
- The parental FLV strain showed minimal spleen-focus assay activity in BALB/c mice, with recessive susceptibility observed in F(1) hybrids.
- The mutant FLV strain exhibited high activity in BALB/c mice, comparable to DBA/2 and ICR/Ha mice.
- Susceptibility to the mutant FLV strain was found to be dominant in (C57BL/6 x DBA/2) F(1) mice.
Conclusions:
- A distinct mutant FLV strain with altered pathogenicity and host tropism has been identified.
- The genetic control of susceptibility to FLV differs significantly between the parental and mutant strains.
- This mutant FLV provides a valuable tool for studying viral pathogenesis and host-genetics of Friend disease.