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Bound water in biology.

C F Hazlewood

    Acta Biochimica Et Biophysica; Academiae Scientiarum Hungaricae
    |January 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Water diffusion in rat skeletal muscle is anisotropic, with a higher spin-diffusion coefficient (Ds) parallel to muscle fibers. This suggests a significant portion of cellular water is bound to proteins, not freely diffusing.

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    Area of Science:

    • Biophysics
    • Physiology
    • Nuclear Magnetic Resonance

    Background:

    • Water diffusion in biological tissues is crucial for cellular function.
    • Skeletal muscle exhibits complex structural organization influencing molecular transport.
    • Pulsed nuclear magnetic resonance (NMR) is a powerful tool for probing molecular dynamics.

    Purpose of the Study:

    • To investigate the spin-diffusion coefficient (Ds) of water protons in rat skeletal muscle.
    • To determine the anisotropy of water diffusion relative to the muscle fiber axis.
    • To interpret diffusion behavior using a biophysical model.

    Main Methods:

    • Pulsed nuclear magnetic resonance (NMR) techniques were employed.
    • The spin-diffusion coefficient (Ds) of water was measured in rat skeletal muscle.

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  • Measurements were conducted at different angles (0, 45, and 90 degrees) relative to the fiber axis.

    • Main Results:

    • Anisotropic water diffusion was observed, with Ds(0)/Ds(90) = 1.39.
    • The spin-diffusion coefficient parallel to the fiber axis, Ds(0), was 1.39 x 10(-5) cm2/sec.
    • A significant reduction in Ds was found parallel to the filament axes.

    Conclusions:

    • Geometrical obstruction models for "free" water diffusion are insufficient.
    • A substantial fraction of cellular water is bound to proteins, reducing its diffusion.
    • Cellular water behaves differently from water in a dilute solution.