[Immunochemical analysis of oligosaccharide fragments of O-specific Shigella flexneri polysaccharides]

Comparison of inhibitory properties of several synthetic oligosaccharides related to Sh. flexneri O-specific polysaccharides, namely Glc alpha 1-3Rha alpha 1-OMe, Glc alpha 1-3Rha alpha 1-2Rha alpha 1-OMe, Rha alpha 1-2(Glc alpha 1-3)Rha alpha 1-OMe, GlcNAc beta 1-2(Glc alpha 1-3)Rha alpha 1-OMe, and GlcNAc beta 1-2(Glc alpha 1-3) Rha alpha 1-2Rha alpha 1-OMe, using passive haemagglutination reaction demonstrated the tetrasaccharide to possess the highest activity in V; 7,8-anti-7,8 immune system. Among the oligosaccharides under study, only Rha alpha 1-2(Glc alpha 1-3)Rha alpha 1-OMe exhibited moderate anti-V activity.