A 10 microliter indirect ultramicro ELISA for detection of monoclonal antibodies against human alphafetoprotein

An ultramicro ELISA, which uses 10 microliter of reaction volume developed for quantitation of human alphafetoprotein (AFP), has been adapted to use in screening hybridoma products in order to take advantage of the efficiency and sensitivity of this system. Positive samples were detected with a low background level.