[Transcriptional complex of hepatocytes during cholinergic stimulation]

Injections of carbamyl choline into rats stimulated RNA synthesis in liver tissue involving initially the ribosomal RNA. The experiments with chromatin isolated from hepatocytes showed that carbamyl choline induced the matrix activation. During incubation of the isolated nuclei in the medium of high ionic strength (0.4 M (NH4)2SO4) containing alpha-amanitine and the enzyme components of the transcriptional complex, the RNA polymerase I was only activated. An increase in the parasympathetic innervation tonus, simulated by cholinomimetics, was accompanied by transcriptional activation with simultaneous derepression of a certain part of genome, including the genes responsible for synthesis of DNA-dependent RNA polymerases.