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Updated: Aug 16, 2026

A Lectin HPLC Method to Enrich Selectively-glycosylated Peptides from Complex Biological Samples
Published on: October 1, 2009
Human serum contains a lectin which inhibits hepatic uptake of glycoproteins
Abstract:
Human serum will inhibit the specific uptake of N-acetylglucosamine-terminated glycoproteins by isolated rat hepatic sinusoidal cells. The serum inhibitors are not glycoproteins that bind to the hepatic mannose/N-acetylglucosamine receptor but have the properties of lectins which bind to mannose/N-acetylglucosamine-terminated glycoproteins. They can be isolated from serum by affinity chromatography. The predominant lectin, with a molecular mass of less than 35 kDa, will inhibit the sinusoidal cell uptake of glycoproteins in vitro.
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