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Single acetylcholine-activated channel currents in developing muscle cells.

S A Siegelbaum, A Trautmann, J Koenig

    Developmental Biology
    |August 1, 1984
    PubMed
    Summary
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    Acetylcholine-activated ion channels in developing rat muscle cells show two conductance classes. Channel density increases as myoblasts fuse into myotubes, with properties remaining consistent.

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biophysics

    Background:

    • Acetylcholine-activated (ACh) ion channels are crucial for neuromuscular transmission.
    • Understanding their development in muscle cells is key to grasping muscle maturation and function.

    Purpose of the Study:

    • To investigate the properties of single ACh-activated ion channels in developing rat myoblasts and myotubes.
    • To characterize changes in channel density and type during muscle cell differentiation in vitro.

    Main Methods:

    • Utilized the gigaohm seal patch clamp technique for high-resolution recordings.
    • Cultured rat myoblasts and myotubes in vitro to observe developmental changes.

    Main Results:

    • Identified two main classes of ACh-activated channels: a major 35 pS channel (mean open time 15 ms) and a minor 55 pS channel (mean open time 2-3 ms).

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  • Found functional channels in early myoblasts, with density increasing significantly as myoblasts fuse into myotubes.
  • Observed an increased proportion of larger conductance channels during myotube formation, while conductance and open times within each class remained stable.
  • Demonstrated channel desensitization at high ACh concentrations, with opening rates dependent on agonist concentration but open times independent.

    • Conclusions:

    • ACh-activated ion channel properties are present early in muscle development and undergo significant changes in density and type during differentiation.
    • The observed developmental changes in channel characteristics likely contribute to the functional maturation of the muscle.
    • Channel behavior, including desensitization, aligns with established models of agonist-receptor interactions.