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Insertion element IS1 encodes two structural genes required for its transposition.

Y Machida, C Machida, E Ohtsubo

    Journal of Molecular Biology
    |August 5, 1984
    PubMed
    Summary
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    Insertion element IS1 has two key genes, insA and insB, essential for its role in DNA recombination. This study identified these genes by mutating potential reading frames and testing their ability to mediate plasmid cointegration.

    Area of Science:

    • Molecular biology
    • Genetics
    • Microbial genetics

    Background:

    • Insertion elements (IS) are mobile genetic components found in bacterial genomes.
    • IS1 is a well-studied insertion element involved in various genetic rearrangements.
    • Understanding the functional genes of IS1 is crucial for deciphering its role in recombination.

    Purpose of the Study:

    • To identify the specific genes within insertion element IS1 responsible for mediating plasmid cointegration.
    • To elucidate the genetic basis of IS1-mediated recombination.

    Main Methods:

    • Nucleotide sequence analysis of IS1 to predict potential protein-coding regions (reading frames).
    • Introduction of targeted mutations, including nonsense mutations, into all predicted reading frames of IS1.

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  • Assay of mutant IS1 elements for their ability to mediate cointegration between two plasmids.

    • Main Results:

    • IS1 possesses multiple potential translational reading frames, suggesting complex gene expression.
    • Mutational analysis revealed that only two specific reading frames are essential for IS1 function.
    • These essential genes were identified as insA and insB, both required for IS1-mediated plasmid cointegration.

    Conclusions:

    • Insertion element IS1 encodes at least two essential genes, insA and insB.
    • These genes are critical for the IS1-mediated process of plasmid cointegration.
    • The findings clarify the genetic architecture underlying IS1's role in DNA recombination.