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Related Experiment Videos

Human monoclonal antibodies.

S P Cole, B G Campling, T Atlaw

    Molecular and Cellular Biochemistry
    |June 1, 1984
    PubMed
    Summary
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    Generating human monoclonal antibodies has advanced significantly. The EBV-hybridoma technique offers a superior method for producing specific human monoclonal antibodies by combining existing approaches.

    Area of Science:

    • Immunotechnology
    • Biotechnology
    • Molecular Biology

    Background:

    • Murine monoclonal antibody production technology is highly refined.
    • Human monoclonal antibody production technology is a recent development.
    • Advances are crucial for therapeutic and diagnostic applications.

    Purpose of the Study:

    • To review current methods for human monoclonal antibody production.
    • To compare the advantages and disadvantages of different techniques.
    • To identify the most effective current method for generating specific human monoclonal antibodies.

    Main Methods:

    • Hybridoma technique: Fusion of human B lymphocytes with myeloma/lymphoblastoid cells.
    • EBV immortalization technique: Epstein-Barr virus (EBV) immortalization of B lymphocytes.

    Related Experiment Videos

  • EBV-hybridoma technique: Combination of hybridoma and EBV immortalization methods.

    • Main Results:

    • The hybridoma technique faces limitations in human cell fusion.
    • EBV immortalization provides a method for B cell immortalization.
    • The EBV-hybridoma system integrates benefits of both methods.
    • This combined system overcomes limitations of individual techniques.

    Conclusions:

    • The EBV-hybridoma technique is a promising approach.
    • It offers a method of choice for producing specific human monoclonal antibodies.
    • Further refinement of human monoclonal antibody production is ongoing.