A spino-reticulo-thalamic pathway in the rat: an anatomical study with reference to pain transmission

The axonal tract tracing technique using the transport in both the retrograde and orthograde directions of wheat-germ agglutinin conjugated to horseradish peroxidase permitted the observation of both retrogradely labelled spinal neurons and anterogradely labelled thalamic fiber terminals in the same animal after injections of the compound in the nucleus reticularis gigantocellularis, thus allowing the definition of the spino-reticulo-thalamic pathway which relays in this nucleus in the rat. Results of the present study are in favor of the existence of a pathway originating mostly in the spinal ventral horn and ending in the intralaminar nuclei of the thalamus, in particular in the nucleus center median, after a relay in the nucleus reticularis gigantocellularis. Origin and termination of this pathway seem to be well differentiated from those of the direct spino-thalamic tract. The results are discussed with reference to the possible involvement of this pathway in some aspects of pain transmission. It is suggested, in particular, that the direct spino-thalamic system which relays in the thalamic ventrobasal complex, presents the features required of a structure playing a role in the sensory-discriminative aspects of pain transmission; in contrast, the spino-reticulo thalamic system defined here could be involved in some motor and/or behavioral responses related to pain.